FIG. 3.

Coprecipitation of Xdsh and XARP. Each blastomere of four-cell embryos was injected with 2 to 4 ng of mRNAs, encoding Myc-XARP, Myc-XARPΔDIX, and HA-Xdsh, as indicated. (A) Association of Xdsh and XARP was revealed by immunoprecipitation of Xdsh with anti-HA antibodies followed by Western analysis with anti-Myc antibodies. Myc-XARP, but not Myc-XARPΔDIX, coprecipitated with HA-Xdsh. The same membrane was stripped and probed with anti-HA antibodies. (B) Levels of protein expression are shown in corresponding embryo lysates.