Fig. 3.

SP2509 inhibits PEDV infection in Vero cells. Vero cells were pretreated with SP2509 for 12 h, followed by mock infection or infection with PEDV (MOI = 0.01). 2 h later, cells were further cultured in viral maintenance solution in the absence or presence of SP2509. The cells were assayed for relevant indices at the indicated times after infection. A IC₅₀ curves of the SP2509. B TCID₅₀ analyzed the virus titer. C RT-qPCR analyzed the Relative RNA expression levels of PEDV ORF3. D The expression of PEDV N was analyzed by Western blot. E The inhibitory effect of SP2509 on PEDV N protein was determined by immunofluorescence assay. F IFA fluorescence quantification results. The experiment was performed three times independently. Differences were considered significant at * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001