Fig. 8.

Contrasting Mitochondrial effects of ST-401 on the sensitive SF-539 and the resistant SNB-19 cells. a-b) Representative images of SF-539 cells and SNB-19 cells c-d) treated for 24 h with vehicle (CTR), NOC (100 nM) or ST-401 (100 nM) and stained with mitoTracker to visualize mitochondria. Unbiased quantification of their volume, sphericity compactness and isoperimetric quotient using statistics, data are shown as median of 3 independent experiments. Welch’s t-test analysis resulted in * = p < 0.05 and ** = p < 0.01 compared to CTR. e–f) Measure of the oxygen consumption rate (OCR) and of extracellular acidification response (ECAR) of SF-539 cells and SNB-19 cells (g-h) treated for 24 h with CTR, NOC (100 nM) or ST-401 (100 nM). Treatment with oligomycin (Olig, 1 µM), FCCP (1 µM), antimycinA/rotenone (1 µM) allowed to resolve the mitochondrial health based on the basal OCR, ATP production, proton leak and spare respiratory capacity. Data are shown as mean ± s.d. of 4 independent experiments. Unpaired t-test resulted in * = p < 0.05 and ** = p < 0.01 compared to CTR