Figure 6.

Effect of heat stress on fluorescence intensity of 5hmC in bovine oocytes and embryos. Secondary antibody Alexa Fluor-594 binds to primary antibody 5-hydroxymethylation with red fluorescence. (A) Representative image staining of control group 5hmC at various cell stages of bovine oocyte development. Bar = 50 μm. (B) Representative image staining of heat-stress group 5hmC at various cell stages of bovine oocyte development. Bar = 50 μm. (C) Expression levels of each cell stage in the control group. (D) Expression levels of each cell stage in the heat-stress group. Values with no common superscript lowercase letters mean significant differences between groups (p  <  0.05). (E) Effect of heat stress on the expression level of 5hmC in bovine oocytes and embryos. The asterisk (*) denotes significant disparities between groups (p < 0.05).