Table 1.
Concentration of the compounds tentatively identified in EVOO samples analyzed.
| Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | Sample 7 | Sample 8 | Sample 9 | Sample 10 | Sample 11 | Sample 12 | Sample 13 | Sample 14 | Sample 15 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PDO | PG | PG | MG | PG | PG | PG | PG | PG | MG | PG | PG | MG | MG | MG | PG |
| Variety | Hojiblanca | Picudo | Picual | Chorreao | Hojiblanca | Manzanillo | Picudo | Chorreao | Picual | Hojiblanca | Hojiblanca | Picual | Picual | Coupage | Picual |
| Concentration of the compounds expressed as µg of compounds g−1 of EVOO | |||||||||||||||
| DiHyGli | 1.4 ± 0.2 | 1.0 ± 0.1 | 0.67 ± 0.03 | 0.10 ± 0.01 | 0.13 ± 0.01 | 0.09 ± 0.01 | 0.25 ± 0.02 | 0.12 ± 0.01 | 0.25 ± 0.02 | 0.38 ± 0.04 | 0.47 ± 0.04 | 0.06 ± 0.01 | 0.04 ± 0.01 | 0.18 ± 0.01 | 0.5 ± 0.1 |
| ForP | 1.6 ± 0.2 | 7.8 ± 0.1 | 0.53 ± 0.04 | 0.010 ± 0.004 | 0.77 ± 0.03 | 0.08 ± 0.01 | 0.68 ± 0.04 | 2.07 ± 0.03 | 1.1 ± 0.2 | 0.67 ± 0.04 | 1.4 ± 0.1 | 0.009 ± 0.002 | 0.20 ± 0.02 | 0.31 ± 0.04 | 0.9 ± 0.1 |
| TMP-Ac | 2.4 ± 0.3 | 2.83 ± 0.2 | 0.35 ± 0.04 | 2.4 ± 0.1 | 1.04 ± 0.05 | 2.6 ± 0.1 | 1.7 ± 0.1 | 5.4 ± 0.3 | 2.0 ± 0.2 | 0.6 ± 0.1 | 0.57 ± 0.04 | 1.70 ± 0.04 | 0.54 ± 0.02 | 1.8 ± 0.1 | 0.8 ± 0.1 |
| DEDA-Ac | 0.5 ± 0.1 | 0.38 ± 0.05 | 0.88 ± 0.06 | 1.4 ± 0.1 | 0.08 ± 0.01 | 1.3 ± 0.1 | 0.9 ± 0.1 | 1.34 ± 0.04 | 0.83 ± 0.02 | 0.25 ± 0.04 | 0.22 ± 0.01 | 0.12 ± 0.02 | 0.19 ± 0.02 | 0.49 ± 0.04 | 0.49 ± 0.02 |
| Ac-Pin | 0.3 ± 0.0 | 0.50 ± 0.01 | 0.026 ± 0.004 | 0.068 ± 0.002 | 0.78 ± 0.02 | 0.12 ± 0.01 | 0.033 ± 0.004 | 0.164 ± 0.005 | 0.070 ± 0.003 | 0.18 ± 0.01 | 0.282 ± 0.004 | 0.20 ± 0.01 | 2.7 ± 0.01 | 0.82 ± 0.02 | 0.17 ± 0.01 |
| Api | 1.3 ± 0.1 | 1.56 ± 0.1 | 0.28 ± 0.02 | 0.70 ± 0.04 | 1.27 ± 0.03 | 0.62 ± 0.02 | 0.95 ± 0.04 | 0.13 ± 0.01 | 0.52 ± 0.05 | 1.0 ± 0.1 | 0.47 ± 0.03 | 0.64 ± 0.04 | 0.77 ± 0.01 | 0.8 ± 0.1 | 1.39 ± 0.03 |
| DEDA | <LOQ | <LOQ | 0.82 ± 0.04 | 0.41 ± 0.02 | 1.5 ± 0.1 | 0.8 ± 0.1 | 2.2 ± 0.1 | 5.1 ± 0.1 | 4 ± 1 | 2.9 ± 0.1 | 0.30 ± 0.02 | 0.39 ± 0.04 | 0.35 ± 0.05 | 0.5 ± 0.1 | 4.2 ± 0.2 |
| EA 1 | 2.4 ± 0.1 | 3.2 ± 0.1 | 0.36 ± 0.02 | 0.77 ± 0.02 | 1.4 ± 0.3 | 1.2 ± 0.4 | 0.9 ± 0.1 | 0.7 ± 0.2 | 1.4 ± 0.1 | 1.1 ± 0.4 | 1.9 ± 0.1 | 1.4 ± 0.2 | 0.4 ± 0.1 | 1.2 ± 0.1 | 0.9 ± 0.1 |
| EA 2 | 37.1 ± 1.0 | 28 ± 2 | 34 ± 2 | 51 ± 2 | 38 ± 2 | 21 ± 1 | 45 ± 3 | 56 ± 3 | 45 ± 1 | 64 ± 5 | 115 ± 3 | 79 ± 2 | 6.5 ± 0.3 | 26 ± 1 | 15.0 ± 0.3 |
| Hy-D-Li-Agl | 79 ± 3 | 63 ± 2 | 199 ± 4 | 387 ± 9 | 37 ± 2 | 341 ± 4 | 111 ± 1 | 232 ± 7 | 104 ± 3 | 55 ± 2 | 90 ± 4 | 43 ± 1 | 93 ± 3 | 83 ± 3 | 58 ± 1 |
| HyDec Ol Ag (I1) | 1.4 ± 0.2 | 0.9 ± 0.1 | 1.5 ± 0.1 | 3.0 ± 0.1 | 1.2 ± 0.1 | 2.2 ± 0.2 | 1.8 ± 0.1 | 2.4 ± 0.1 | 1.4 ± 0.1 | 5.4 ± 0.3 | 1.22 ± 0.04 | 0.61 ± 0.03 | 0.66 ± 0.04 | 1.1 ± 0.1 | 3.0 ± 0.3 |
| HyDec Ol Ag (I2) | 3.3 ± 0.4 | 3.9 ± 0.2 | 3.9 ± 0.2 | 4.9 ± 0.4 | 2.2 ± 0.3 | 8.3 ± 0.2 | 7.6 ± 0.2 | 14 ± 1 | 5.5 ± 0.5 | 1.8 ± 0.1 | 1.9 ± 0.1 | 2.1 ± 0.1 | 4.2 ± 0.2 | 4.7 ± 0.2 | 2.4 ± 0.1 |
| Hy-EA | 2.4 ± 0.4 | 4.2 ± 0.1 | 1.2 ± 0.1 | 0.28 ± 0.04 | 2.5 ± 0.2 | 1.49 ± 0.04 | 1.6 ± 0.1 | 2.2 ± 0.1 | 2.6 ± 0.3 | 0.7 ± 0.1 | 1.8 ± 0.1 | 1.56 ± 0.04 | 0.6 ± 0.1 | 2.3 ± 0.2 | 0.62 ± 0.03 |
| Hy-Ol-Ag 1 | 0.9 ± 0.1 | 1.4 ± 0.2 | 2.8 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0.1 | 1.1 ± 0.1 | 0.6 ± 0.1 | 1.9 ± 0.1 | 5.2 ± 0.4 | 0.3 ± 0.1 | 1.1 ± 0.1 | 3.2 ± 0.3 | 0.09 ± 0.03 | 0.6 ± 0.1 | 0.9 ± 0.1 |
| Hy-Ol-Ag 2 | 0.5 ± 0.1 | 1.2 ± 0.1 | 2.6 ± 0.2 | 0.6 ± 0.1 | <LOD | 0.8 ± 0.1 | 0.34 ± 0.04 | 1.7 ± 0.1 | 5.2 ± 0.5 | <LOQ | 0.5 ± 0.1 | 3.1 ± 0.1 | 0.04 ± 0.01 | 0.34 ± 0.04 | 0.42 ± 0.03 |
| Hy-Ol-Ag 3 | 1.4 ± 0.2 | 2.2 ± 0.1 | 5.4 ± 0.3 | 1.5 ± 0.2 | 1.02 ± 0.03 | 4.1 ± 0.2 | 3.8 ± 0.1 | 4.2 ± 0.3 | 7 ± 1 | 1.6 ± 0.2 | 2.4 ± 0.1 | 3.3 ± 0.1 | 0.87 ± 0.02 | 2.8 ± 0.2 | 1.3 ± 0.1 |
| Hy-Ol-Ag 4 | 0.7 ± 0.1 | 1.24 ± 0.04 | 0.07 ± 0.02 | 0.16 ± 0.01 | 0.36 ± 0.01 | 0.37 ± 0.03 | 0.77 ± 0.02 | 0.60 ± 0.05 | 0.7 ± 0.1 | 0.28 ± 0.04 | 0.31 ± 0.01 | 0.57 ± 0.04 | 0.16 ± 0.01 | 0.4 ± 0.1 | 0.16 ± 0.01 |
| Hyty | 2.1 ± 0.2 | 2.8 ± 0.1 | 6.5 ± 0.1 | 3.5 ± 0.1 | 2.3 ± 0.1 | 3.7 ± 0.2 | 7.7 ± 0.1 | 9.3 ± 0.2 | 12 ± 2 | 3.3 ± 0.2 | 7.1 ± 0.1 | 4.4 ± 0.2 | 3.9 ± 0.2 | 3.8 ± 0.1 | 5.6 ± 0.1 |
| Hyty-Ac | <LOD | <LOD | 0.93 ± 0.02 | <LOD | 0.02 ± 0.01 | <LOD | <LOD | <LOD | 0.12 ± 0.02 | <LOD | 0.118 ± 0.004 | 0.34 ± 0.03 | 1.1 ± 0.1 | 0.71 ± 0.03 | 0.54 ± 0.02 |
| Li-Ag 1 | 39 ± 5 | 26 ± 2 | 4 ± 0.2 | 7 ± 1 | 7.5 ± 0.2 | 31 ± 3 | 8 ± 1 | 5.6 ± 0.3 | 58 ± 4 | 6 ± 1 | 21 ± 1 | 39 ± 2 | 0.23 ± 0.01 | 6.3 ± 0.2 | 7.1 ± 0.3 |
| Li-Ag 2 | 17 ± 3 | 12 ± 1 | 9.2 ± 0.4 | 10.8 ± 0.4 | 8.8 ± 0.4 | 21 ± 2 | 15 ± 1 | 6.4 ± 0.3 | 33 ± 2 | 11 ± 1 | 8 ± 1 | 17 ± 0.5 | 0.47 ± 0.02 | 6.3 ± 0.2 | 6.5 ± 0.3 |
| Li-Ag 3 | 10 ± 1 | 8.0 ± 0.4 | 8 ± 1 | 8.1 ± 0.5 | 6.2 ± 0.6 | 15 ± 1 | 10 ± 1 | 5 ± 1 | 16 ± 2 | 8 ± 1 | 9 ± 2 | 12 ± 1 | 0.14 ± 0.04 | 3.82 ± 0.04 | 4.2 ± 0.1 |
| Li-Ag 4 | 54 ± 5 | 74 ± 2 | 246 ± 5 | 118 ± 4 | 45 ± 1 | 348 ± 4 | 127 ± 4 | 94 ± 2 | 219 ± 6 | 74 ± 1 | 74 ± 1 | 50 ± 3 | 14 ± 2 | 61 ± 1 | 49 ± 1 |
| Li-Ag 5 | 4.5 ± 0.2 | 3.9 ± 0.1 | 33 ± 0.1 | 7.9 ± 0.7 | 4.7 ± 0.2 | 9 ± 1 | 7.5 ± 0.3 | 7.6 ± 0.2 | 9.6 ± 0.4 | 5.8 ± 0.3 | 6 ± 1 | 6.3 ± 0.2 | 1.12 ± 0.03 | 5.6 ± 0.2 | <LOQ |
| Li-Ag 6 | 6.6 ± 0.2 | 5.3 ± 0.1 | 11.9 ± 0.4 | 13 ± 2 | 7.8 ± 0.3 | 21 ± 2 | 6.7 ± 0.3 | 13 ± 1 | 11 ± 1 | 10 ± 1 | 6.3 ± 0.4 | 9 ± 1 | 4.2 ± 0.3 | 10.9 ± 0.3 | 9.1 ± 0.3 |
| Li-Ag 7 | 2.4 ± 0.1 | 1.9 ± 0.1 | 1.81 ± 0.04 | 2.8 ± 0.2 | 2.1 ± 0.1 | 3.0 ± 0.1 | 3.1 ± 0.2 | 2.2 ± 0.1 | 2.7 ± 0.3 | 2.6 ± 0.2 | 1.4 ± 0.2 | 2.3 ± 0.1 | 0.13 ± 0.02 | 1.58 ± 0.58 | 2.4 ± 0.1 |
| Lut | 2.3 ± 0.3 | 2.7 ± 0.1 | 1.00 ± 0.04 | 1.00 ± 0.03 | 2.34 ± 0.03 | 0.80 ± 0.03 | 1.0 ± 0.1 | 0.55 ± 0.02 | 1.9 ± 0.1 | 1.9 ± 0.1 | 1.10 ± 0.05 | 1.2 ± 0.1 | 2.14 ± 0.03 | 1.5 ± 0.1 | 2.8 ± 0.1 |
| Me-Ol-Ag 1 | <LOQ | <LOQ | 1.6 ± 0.1 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | 1.2 ± 0.2 | 0.7 ± 0.1 | 2.58 ± 0.05 | 0.4 ± 0.05 | <LOQ | <LOQ | <LOQ |
| Me-Ol-Ag 2 | <LOQ | <LOQ | 0.7 ± 0.2 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | 0.8 ± 0.1 | 0.11 ± 0.03 | 1.9 ± 0.1 | 0.20 ± 0.01 | <LOQ | <LOQ | <LOQ |
| Me-Ol-Ag 3 | <LOQ | <LOQ | 1.2 ± 0.1 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | 0.6 ± 0.1 | <LOQ | 1.3 ± 0.1 | <LOQ | <LOQ | <LOQ | <LOQ |
| Me-Ol-Ag 4 | <LOQ | 0.32 ± 0.01 | 0.64 ± 0.2 | 0.16 ± 0.02 | 0.21 ± 0.03 | <LOQ | 0.14 ± 0.01 | <LOQ | 0.15 ± 0.04 | 0.29 ± 0.04 | 0.6 ± 0.1 | 0.07 ± 0.03 | 0.36 ± 0.04 | 0.32 ± 0.03 | <LOQ |
| Me-Ol-Ag 5 | 0.30 ± 0.03 | 0.2 ± 0.01 | 24 ± 2 | 0.9 ± 0.1 | <LOQ | 0.39 ± 0.02 | 0.86 ± 0.03 | <LOQ | 3.7 ± 0.3 | 2.8 ± 0.1 | 5.5 ± 0.1 | 1.39 ± 0.04 | 1.3 ± 0.1 | 1.9 ± 0.1 | 0.24 ± 0.02 |
| Me-Ol-Ag 6 | 0.13 ± 0.04 | <LOQ | 6.6 ± 0.3 | 0.6 ± 0.1 | <LOQ | 0.14 ± 0.01 | 0.41 ± 0.02 | <LOQ | 1.6 ± 0.1 | 1.7 ± 0.1 | 2.8 ± 0.1 | 0.71 ± 0.04 | 1.04 ± 0.04 | 1.3 ± 0.1 | <LOQ |
| Ol-Ag 1 | 9.7 ± 0.5 | 17 ± 1 | 8 ± 1 | 6.2 ± 0.5 | 3.5 ± 0.2 | 7.7 ± 0.4 | 5.7 ± 0.4 | 5 ± 1 | 23 ± 3 | 4.8 ± 0.5 | 7.0 ± 0.3 | 2.6 ± 0.3 | 0.7 ± 0.1 | 3.4 ± 0.3 | 3.7 ± 0.1 |
| Ol-Ag 2 | 82 ± 3 | 51 ± 3 | 777 ± 4 | 320 ± 7 | 46 ± 1 | 422 ± 5 | 193 ± 6 | 236 ± 9 | 569 ± 8 | 95 ± 2 | 131 ± 3 | 114 ± 3 | 84 ± 4 | 226 ± 6 | 122 ± 11 |
| Ol-Ag 3 | 43 ± 1 | 33 ± 2 | 59 ± 1 | 57 ± 2 | 30 ± 2 | 64 ± 2 | 42 ± 2 | 44 ± 2 | 80 ± 4 | 30 ± 1 | 63 ± 1 | 41 ± 2 | 6.2 ± 0.1 | 43 ± 1 | 31 ± 1 |
| Ol-Ag 4 | 6.0 ± 0.2 | 5.5 ± 0.3 | 5.3 ± 0.4 | 5.1 ± 0.2 | 3.6 ± 0.1 | 4.1 ± 0.1 | 4.6 ± 0.5 | 4.9 ± 0.1 | 7 ± 1 | 5.2 ± 0.5 | 4.2 ± 0.1 | 5.0 ± 0.2 | 0.82 ± 0.02 | 2.3 ± 0.1 | 4.1 ± 0.2 |
| Pin | 0.20 ± 0.01 | 0.25 ± 0.01 | 0.54 ± 0.02 | 0.16 ± 0.01 | 0.49 ± 0.01 | 0.18 ± 0.01 | 0.153 ± 0.004 | 0.252 ± 0.002 | 0.54 ± 0.02 | 0.27 ± 0.01 | 0.207 ± 0.01 | 0.66 ± 0.01 | 1.33 ± 0.03 | 0.79 ± 0.03 | 1.08 ± 0.02 |
| QuiAc | 0.08 ± 0.01 | 0.044 ± 0.003 | 3.2 ± 0.1 | 0.036 ± 0.001 | 0.019 ± 0.002 | 0.008 ± 0.002 | <LOD | <LOD | 0.9 ± 0.1 | 0.52 ± 0.03 | 1.2 ± 0.1 | 0.21 ± 0.01 | <LOD | 0.21 ± 0.03 | 0.78 ± 0.01 |
| Syr | 0.29 ± 0.01 | 0.30 ± 0.01 | 0.17 ± 0.01 | 0.19 ± 0.01 | 0.391 ± 0.002 | 0.228 ± 0.001 | 0.220 ± 0.005 | 0.127 ± 0.004 | 0.122 ± 0.003 | 0.40 ± 0.01 | 0.30 ± 0.01 | 0.22 ± 0.01 | 1.14 ± 0.03 | 0.48 ± 0.01 | 0.28 ± 0.01 |
| Tyr | 1.4 ± 0.1 | 1.3 ± 0.1 | 5.5 ± 0.3 | 3.4 ± 0.1 | 2.0 ± 0.1 | 2.4 ± 0.1 | 5.7 ± 0.1 | 10.8 ± 0.4 | 6 ± 1 | 3.3 ± 0.1 | 3.5 ± 0.1 | 2.2 ± 0.1 | 2.5 ± 0.2 | 1.8 ± 0.1 | 3.9 ± 0.1 |
| Van | 0.5 ± 0.1 | 0.65 ± 0.02 | 0.19 ± 0.01 | 0.25 ± 0.01 | 0.50 ± 0.02 | 0.26 ± 0.01 | 0.21 ± 0.02 | 0.19 ± 0.01 | 0.6 ± 0.1 | 0.54 ± 0.04 | 0.14 ± 0.01 | 0.24 ± 0.01 | 0.45 ± 0.03 | 0.39 ± 0.03 | 0.38 ± 0.01 |
| Total | 418 | 369 | 1471 | 1021 | 263 | 1341 | 619 | 775 | 1245 | 404 | 579 | 451 | 239 | 511 | 346 |
PDO, Protective Denomination of Origin; PG, Poniente de Granada; MG, Montes de Granada; Coupage: mix of picual, arbequino and hojiblanca; LOQ, Limit of quantification; LOD, Limit of Detection. Full name of abbreviations can be found in Table S1.