TABLE 2.
Rescue of precocious dauer formation by p14GFP and p14B2GFP in lin-14(n179ts) and lin-14(ma135) mutants
| Straina | Cultureb | % Dauer larvae arrested at L1 molt (n)
|
|
|---|---|---|---|
| GFP− | GFP+ | ||
| VT886 (ma135; maEx167) | 886-1 | 85 (148) | 8 (116) |
| 886-2 | 70 (67) | 2 (82) | |
| VT887 @ 25°C (n179; maEx168) | 887-1 | 67 (21) | 1 (101) |
| 887-2 | 69 (150) | 0 (63) | |
| VT888 (ma135; maEx168) | 888-1 | 81 (100) | 14 (131) |
| 888-2 | 76 (66) | 12 (67) | |
These transgenic lines carry extrachromosomal arrays (maEx167 [p14GFP] or maEx168 [p14B2GFP]) that are frequently lost at meiosis, generating a mixture of nontransgenic (GFP−) and transgenic (GFP+) animals. Dauer larvae were harvested from starved plate cultures and separated into nontransgenic (GFP−) and transgenic (GFP+) classes. The percentage of the dauer larvae in each class that were arrested at the L1 molt was determined as described in Materials and Methods. n, total number of dauer larvae scored; all dauer larvae not arrested at the L1 molt were arrested at the L2 molt, as in the wild type.
For each strain, two starved plates were scored separately.