Impact of carnosic acid on host cell viability and HSV-2 replication. (A), Impact of 24 h carnosic acid treatment on Vero cell viability measured by MTT assay. (B), Impact of 24 h carnosic acid treatment on HSV-2 replication in Vero cells measured by direct qPCR. (C), Impact of 30 min carnosic acid treatment on Vero cell viability measured by MTT assay. (D), Impact of 30 min carnosic acid treatment of HSV-2 virions on HSV-2 replication measured by direct qPCR 24 h post-infection. (E), Impact of 24 h carnosic acid treatment on HeLa cell viability measured by MTT assay. (F), Impact of 24 h carnosic acid treatment on HSV-2 replication in HeLa cells measured by direct qPCR. (G), Impact of 30 min carnosic acid treatment on HeLa cell viability measured by MTT assay. (H), Impact of 30 min carnosic acid treatment of HSV-2 virions on HSV-2 replication measured by direct qPCR 24 h post-infection. MTT data are mean ± SD (n = 4), and qPCR data are mean ± SD (n = 3). Statistical comparisons of treated vs. untreated samples were performed by one-way ANOVA analysis corrected by Dunnett’s multiple comparison test. *: p < 0.05.