Figure 7.

Mitochondrial effects of carnosic acid. (A), High-resolution respirometry of carnosic acid-treated HeLa cells. ROUTINE-, ATP-linked respiration, LEAK, and maximal capacity of the electron transport system (ETS) were significantly attenuated in carnosic acid-treated HeLa cells in comparison with control. The comparison of oxygen consumption values was performed by the Mann–Whitney U test ** p < 0.01. (B), Impact of inhibition of oxidative phosphorylation on HSV-2 replication. After HSV-2 infection, culture media were replaced with a media containing oligomycin at a 16–0 μg/mL concentration. HeLa cells were incubated for 24 h, 37 °C, 5%CO₂. HSV-2 replication was measured by direct qPCR. Statistical analysis of qPCR data was performed by one-way ANOVA analysis corrected by Dunnett’s multiple comparison test. **: p < 0.001.