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. 2024 Apr 26;25(9):4757. doi: 10.3390/ijms25094757

Figure 5.

Figure 5

Differential signalling of NR2A or NR2B-containing NMDAR in primary cultures of neurons treated with Aβ1-42. Cortical and hippocampal primary cultures of neurons were transfected with siRNA for NR2A (blue), siRNA for NR2B (violet) or vehicle (black) and treated with Aβ1-42 (1 µM) or vehicle for 48 h. After, cells were stimulated with a range of NMDA concentrations (10 nM to 300 µM) or vehicle for 10 min in MAPK phosphorylation assays or a few seconds when detecting calcium release. ERK1/2 phosphorylation was analysed using an AlphaScreen®SureFire® kit (Perkin Elmer) (A–D). Calcium release was detected by Fluo-4 DirectTM Calcium Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) in a NIVO Multimode reader (Thermo Fisher) (E–H). Values are the mean ± S.E.M. of 8 independent experiments performed in triplicates.