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. 2024 May 3;25(9):4994. doi: 10.3390/ijms25094994

Figure 2.

Figure 2

Effect of Mg2+ on cell migration. (AD) HaCaT cells were cultured in 24 well plates until confluent and scratched with a pipette tip. Then, the cells were cultured in 0.5% FBS medium supplemented with 0.8–10.8 mM MgCl2 (A,B) or 5.8 mM MgSO4 (C,D). (A) The typical images of the cells with DMEM containing 0.8 mM MgCl2 and 5.8 mM MgCl2 are presented. (B) The scratched area was examined as a wound healing area (mm2) in each time point. n = 4. (C) Typical images of the cells with 5.8 mM MgSO4 are presented. (D) The scratched area was examined as a wound healing area (mm2) in 18 h. n = 4. ** p < 0.01, * p < 0.05, NS p > 0.05 vs. Control. Yellow shows the wounded area. Blue shows the initial wounded area.