Figure 2.

Hematoxylin staining of ASC cultures after 1 day (A) and 7 days (B) of growth in different conditions. Control ASCs and those grown in a conditioned medium from OECs and SCs are illustrated in the top row of each panel. In corresponding samples, 1 μM melatonin was added (bottom row of each panel). It can be stated that, regardless of the presence of melatonin, denser cell populations are present after 7 days of culture if they are kept in their basal medium, in which they show the typical fibroblast-like shape (first column). Less dense populations were observed in the presence of glial conditioned media (second and third column), especially on day 7. In these conditions, more elongated cytoplasmic prolongations can be observed (arrowheads), especially in the presence of melatonin (double arrowheads).