FIG. 2.

Localization of TS protein in MCMV-infected quiescent NIH 3T3 cells by indirect immunofluorescence. NIH 3T3 cells grown on coverslips were growth arrested in 0.5% calf serum for 48 h and then infected with MCMV (MOI of 0.5 PFU/cell) or UV-irradiated MCMV or mock infected. At 48 h p.i., cells were fixed with paraformaldehyde, permeabilized, and costained with MCMV IE1 (A) and TS (B) antibodies, as described in Materials and Methods. The merged image is shown in panel C. The immunolocalization experiments were repeated three times, and representative results are presented.