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. 2024 Apr 26;25(9):4735. doi: 10.3390/ijms25094735

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of cancerous ascites fluid on rat H9c2 cardiomyoblasts. H9c2 cells were treated with CM containing ascites obtained from RCN9 CRC cells for 48 h. (A) MtHRs. (B) MMP. (C) MtVol. (D) Number of apoptotic cells. (E) PARP cleavage. (F) autophagy. (G) Levels of the autophagy-associated proteins. Right panels: fluorescent images (A–C,F); semi-quantified graph (G). Scale bar, 50 μm. Statistical significance was calculated using an ordinary ANOVA. Asterisk, p value. CaCM, cancerous ascites mixed culture medium; C, control medium; PARP, poly ADP-ribose polymerase; ATG5, autophagy-related gene 5; LC3, microtubule-associated protein 1A/1B-light chain 3; mtHR, mitochondrial hydroxylase; MMP, mitochondrial membrane potential; mtVol, mitochondrial volume.