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. 2024 Apr 30;25(9):4935. doi: 10.3390/ijms25094935

Figure 3.

Figure 3

Comparison of the levels of serum anti-JMJD6 antibodies (s-JMJD6-Abs) in healthy donors (HDs) and patients with esophageal cancer (EC), gastric cancer (GC), lung cancer (LC), and mammary cancer (MC). (a) The figure shows the levels of s-JMJD6-Abs examined by amplified luminescence proximity homogeneous assay-linked immunosorbent assay (AlphaLISA). Antibody levels are represented by Alpha photon counts and shown in a scatter dot plot; the horizontal lines represent medians and 25th and 75th percentiles. The p-values were calculated using the Kruskal–Wallis test. (b–e) The abilities of s-JMJD6-Ab levels to detect EC (b), GC (c), LC (d), and MC (e) were assessed by receiver operating characteristic (ROC) curve analysis. The numbers in the figures are cutoff values for the s-JMJD6-Ab levels, sensitivity (left), specificity (right), the area under the curve (AUC), and 95% confidence interval (95% CI), respectively. (f,g) The overall survival in patients with EC and GC between the s-JMJD6-Ab-positive (JMJD6-Ab+) and s-JMJD6-Ab-negative (JMJD6-Ab−) groups (p = 0.0290, p = 0.0970, respectively) was compared and analyzed through a log-rank test. The cutoff values were set at the lowest p-values using X-tile, and the p-values and cutoff values of s-JMJD6-Abs in patients with EC or GC are shown. The values that were lower than cutoff values were considered to be negative, and those of higher-than-cutoff values were positive values. (h,i) The overall survival of patients was stratified by their PD-L1 status (PD-L1-positive (PD-L1+) or PD-L1-negative (PD-L1−)) alone (h) and in combination with the s-JMJD6-Ab levels (i). (j) The overall survival status of patients with EC was assessed according to JMJD6 mRNA expression levels (JMJD6-positive group, JMJD6+; or JMJD6-negative group, JMJD6−) collected from the TCGA database. The cutoff value was set at the lowest p-value from X-tile, and the cutoff value and p-value for JMJD6 mRNA expression levels in patients with EC are shown. Values below the cutoff were considered negative and values above the cutoff were considered positive.