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. 2024 Apr 30;25(9):4874. doi: 10.3390/ijms25094874

Figure 1.

Figure 1

Accumulation of 8-oxoG DNA lesions in serum-deprived A549 lung adenocarcinoma cells treated with hydrogen peroxide at the indicated times. (A) Immunofluorescence of A549 cells treated with hydrogen peroxide at different time points in control (siCt) or VRK1-depleted cells stained by IF and detected using confocal microscopy. The boxed cells are individually shown on the right. (B) Quantification of the levels of 8-oxoG induced by hydrogen peroxide in the experiment shown in part a. VRK1 depletion with two different siVRK1s enhances significantly by itself the accumulation of 8-oxoG lesions in DNA. (C) Immunoblot showing VRK1 levels. β-actin was used as a loading control. * p < 0.05; *** p < 0.001; a.u.: arbitrary units; siCt: siControl; siV-02: siVRK1-02; siV-03: siVRK1-03. NT: non-treated. Experiments were independently performed three times.