Figure 3.

VRK1 depletion impairs the acetylation of H4K16 in A549 lung adenocarcinoma cells exposed to hydrogen peroxide. (A). Image panels showing H4K16ac levels stained by IF and detected using confocal microscopy. DAPI was used to stain the nuclei and VRK1 for knockdown control. VRK1 was depleted using two siRNAs (siVRK1-02 and siVRK1-03) for 72 h. Cells were treated with 200 µM H₂O₂ for 15 and 30 min. (B) Quantification of H4K16ac fluorescence per nuclear area (a.u.: arbitrary units) of 50 cells (per condition) represented in a boxplot. Scale bar = 15 µm; * p < 0.05; *** p < 0.001; a.u.: arbitrary units; NT: non-treated; siCt: siControl; siV-02: siVRK1-02; siV-03: siVRK1-03. VRK1 levels were detected by immunoblot and are shown at the bottom. β-actin was used as a loading control. Experiments were independently performed three times.