Figure 4.

VRK1 depletion impairs the acetylation of H3K9 in A549 lung adenocarcinoma cells exposed to hydrogen peroxide. (A) Image panels showing H3K9ac levels stained by IF and detected using confocal microscopy. VRK1 was depleted using two siRNAs (siVRK1-02 and siVRK1-03) for 72 h. Cells were treated with 200 µM H₂O₂ for 15 and 30 min. (B) Quantification of H4K9ac fluorescence per nuclear area (a.u.) of 50 cells (per condition) represented in a boxplot. (C) VRK1 levels were detected by immunoblot and are shown at the bottom. β-actin was used as a loading control. Scale bar: 20 µm; ** p < 0.01, *** p < 0.001; NT: non-treated; a.u.: arbitrary units; siCt: siControl; siV-02: siVRK1-02; siV-03: siVRK1-03. Experiments were independently performed three times. Box: represent the detail.