Figure 6.

VRK1 depletion facilitates the trimethylation of H3K4 in A549 lung adenocarcinoma cells exposed to hydrogen peroxide. (A) Image panels showing trimethylation of H3K4 levels stained by IF and detected using confocal microscopy. VRK1 was depleted using two siRNAs (siVRK1-02 and siVRK1-03) for 72 h. Cells were treated with 200 µM H₂O₂ for 15 and 30 min. (B) Quantification of H3K4me3 fluorescence per nuclear area (a.u.: arbitrary units) of 50 cells (per condition). (C) VRK1 levels were detected by immunoblot and are shown at the bottom. β-actin was used as a loading control. Scale bar = 15 µm; ** p < 0.01; *** p < 0.001; NT: non-treated; siCt: siControl; siV-02: siVRK1-02; siV-03: siVRK1-03. Experiments were independently performed three times.