Table 1.
Phytochemical | tR (min) | Wavelength (nm) | Slope (b) | Intercept (a) | r | Noise (Sy/x) | LOD (µg/mL) | LOQ (µg/mL) | Instrumental Precision at 0.5 µg/mL (% RSD) |
---|---|---|---|---|---|---|---|---|---|
Catechin | 7.15 | 280 | 0.45 | −0.05 | 0.9981 | 0.015 | 0.10 | 0.34 | 0.98 |
4-HBA | 7.41 | 254 | 6.41 | −0.65 | 0.9932 | 0.030 | 0.01 | 0.05 | 1.34 |
CA | 8.11 | 320 | 1.29 | −0.35 | 0.9924 | 0.033 | 0.08 | 0.25 | 1.87 |
VA | 8.16 | 254 | 3.95 | −0.49 | 0.9912 | 0.034 | 0.03 | 0.09 | 1.29 |
pCA | 9.35 | 254 | 0.83 | −0.07 | 0.9941 | 0.028 | 0.10 | 0.33 | 1.03 |
FA | 9.93 | 254 | 1.96 | −0.02 | 0.9929 | 0.030 | 0.05 | 0.15 | 1.56 |
Naringin | 11.26 | 280 | 2.51 | −0.26 | 0.9978 | 0.016 | 0.02 | 0.06 | 0.36 |
Hesperidin | 11.83 | 280 | 3.22 | −0.31 | 0.9937 | 0.024 | 0.02 | 0.07 | 1.81 |
Quercetin | 15.75 | 360 | 1.49 | −0.99 | 0.9982 | 0.018 | 0.04 | 0.12 | 1.27 |
Naringenin | 16.13 | 280 | 5.73 | −0.34 | 0.9975 | 0.012 | 0.01 | 0.02 | 0.71 |
Hesperetin | 18.11 | 280 | 8.56 | −1.14 | 0.9978 | 0.014 | 0.01 | 0.02 | 1.64 |
Kaemferol | 19.96 | 360 | 2.52 | −1.38 | 0.9981 | 0.026 | 0.03 | 0.10 | 1.91 |
tR = retention time.