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. 2024 Apr 29;13(9):1237. doi: 10.3390/plants13091237

Figure 4.

Figure 4

Overexpression of MsDREB1C in alfalfa affected plant growth. (A) Transcriptional analysis of MsDREB1C in the MsDREB1C overexpression alfalfa lines (OE2, OE8). (B) Plant height of the wild-type (WT) and transgenic plants (OE2, OE8) for 30 days in ‘environment I’, n = 8. ‘Environment I’ represents the conditions in the growth chamber (24 °C/22 °C, 16 h/8 h daytime/nighttime cycles), that is, long-day conditions. (C) Image of the indicated alfalfa lines on Day 30 of the plants from (B). Bar = 3 cm. Branch number (D) and biomass (E) of the plants shown in (C). (F) Plant height of alfalfa plants grown outdoors in ‘environment II’, n = 4. Environment II: Outdoors from 19 September to 19 October 2023, Beijing (39°28′ to 41°03′ north latitude and 115°25′ to 117°35′ east longitude), namely short-day conditions. (G) Image of the indicated alfalfa lines in pots outdoors on Day 30. Bar = 3 cm. Branch number (H) and biomass (I) of the alfalfa plants shown in (G). Notes: The results were analyzed using a one-way ANOVA test. “*” represented p < 0.05, “**” for p < 0.01.