FIG. 7.
Stability of coronavirus defective genomes containing different lengths of poly(A) tail. (A) HCT cells transfected for 4 h with 1 μg of Drep RNAs. (B) 17Cl1 cells transfected for 4 h with 1 μg of MIDI-C RNAs. At various times posttransfection, total intracellular RNA was extracted and analyzed for the presence of Drep RNA by Northern blotting. The times when RNA was harvested are denoted above each panel. (A) Upper panel, lanes 1 to 5, DrepA1 RNA; upper panel, lanes 6 to 10, DrepA5 RNA; lower panel, lanes 1 to 5, DrepA10 RNA; lower panel, lanes 6 to 10, DrepAwt RNA. (B) Upper panel, lanes 1 to 7, MIDI-C A0 RNA; upper panel, lanes 8 to 14, MIDI-C A5 RNA; lower panel, lanes 1 to 7, MIDI-C A10 RNA; lower panel, lanes 8 to 14, MIDI-C Awt RNA.