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. 2024 May 7;20(1):2342592. doi: 10.1080/21645515.2024.2342592

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — During the entire study, mRNA-LNPs were stored at 4°C, and the samples were stored after 3 and 6 months for evaluation of their biological activity. (a-b) Blood was collected from mice at 7 and 14 days after secondary immunization. (c–d) Blood was collected from mice on days 7 and 14 after secondary immunization when stored for up to 3 months. (e–f) Blood was collected from mice at 7 and 14 days after secondary immunization at 6 months of storage. (g-h) Comparison of serum antibody titers at different intervals for four groups of preparations. Data were analyzed through a two-way ANOVA, Data represent the mean ± SD (n = 6, ^ns p > .05, *p < .05, **p < .01, ***p < .001, ****p < .0001).