Figure 3.

NKG2D is the main activating receptor driving ML NK cells against CRC cells. cNK and ML NK cells from healthy donors were incubated with blocking Abs for 30 min before the stimulation with HCT116 cells at 5:1 E:T ratio. a) Representative flow plots and b) summary data of IFN-γ production by cNK and ML NK cells stimulated with HCT116 cells without and with single and combined mAb blockade. Live imaging analysis using incucyte shows killing activity of c) cNK and d) ML NK cells against HCT116 cells in presence of antibody blockade. IgG1 isotype was used as control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.