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. 2024 May 7;16(1):2350151. doi: 10.1080/19490976.2024.2350151

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 8. — Butyrate alleviates intestinal damage caused by hypoxia by down-regulating HIF-1α in NCM460.

(a) Western blot of ZO1, HIF-1α and occludin. Alpha-tubulin is the internal reference protein. (b) Densitometry quantification of occludin level by Western blot (Con: n = 4; CoCl₂: n = 4; NaB: n = 4). (c) Densitometry quantification of ZO-1 level by Western blot (Con: n = 4; CoCl₂: n = 4; NaB: n = 4). (d) Densitometry quantification of HIF-1α level by Western blot (Con: n = 4; CoCl₂: n = 4; NaB: n = 4). (e) mRNA expression of VEGF (Con: n = 3; CoCl₂: n = 3; NaB: n = 3). (f) mRNA expression of BNIP3 (Con: n = 3; CoCl₂: n = 3; NaB: n = 3). (g) mRNA expression of HK2 (Con: n = 3; CoCl₂: n = 3; NaB: n = 3). (h) mRNA expression of PDK1 (Con: n = 3; CoCl₂: n = 3; NaB: n = 3). (i) mRNA expression of LDHA (Con: n = 3; CoCl₂: n = 3; NaB: n = 3). (j) LDHA relative activity (Con: n = 4; CoCl₂: n = 4; NaB: n = 4). (k) Western blot of HIF-1α. Alpha-tubulin is the internal reference protein. (l) Densitometry quantification of HIF-1α level by Western blot (Con: n = 4; CoCl₂: n = 4; lactate: n = 4). “*” stands for significant difference. *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. The p value is calculated by t-test.