FIG. 1.

Binding of mouse brain cell proteins with the JEV 3′-NCR RNA. An RNA-protein binding reaction was carried out using 6 ng of radiolabeled 3′-NCR RNA (597 nucleotides long) and 10 μg of mouse brain cell S100 extract in the presence of different amounts of KCl. The RNA-protein complexes were resolved on a 5% polyacrylamide gel (acrylamide/bisacrylamide ratio, 120:1). (A) Lane 1, free probe; lanes 2, 3, and 4, binding in the presence of 200, 500, and 1,000 ng of poly(I)-poly(C), respectively; lane 5, binding in the presence of 10 μg of yeast tRNA. All of these binding reactions were carried out in the presence of 20 mM KCl. (B) Lane 1, free probe; lanes 2, 3, and 4, binding in the presence of 20, 60, and 200 mM KCl, respectively; lane 5, binding in the presence of 60 mM KCl and 1 ng of unlabeled 3′-SL RNA (101 nucleotides long); lane 6, binding in the presence of 60 mM KCl and 6 ng of unlabeled 3′-SL RNA. All of these binding reactions were carried out in the presence of 200 ng of poly(I)-poly(C).