FIG. 1.

Schematic representation of the transcription plasmid TVT7R(0,0). (A) DNA sequence of the region encompassing the T7 promoter (underlined), transcription start site, and site of RNA cleavage (both indicated with arrowheads). The BbsI recognition sequences are shown in italics, and the nucleotides that remain following excision of the short stuffer fragment by BbsI digestion are shown in bold. Positions of the HDV antigenomic ribozyme (Rz) and T7 terminator sequences are shown. (B) Positioning of PaV cDNAs in the transcription plasmid.