Fig. 4. The 68-1.2 RhCMV vectors elicit MHC-Ia–restricted CD8⁺ T cells to RhPAP.

(A) Three male RMs were inoculated with 68-1.2 RhCMV/RhPAP. CD8⁺ and CD4⁺ T cell responses were determined by ICS in PBMC using an overlapping peptide mix for RhPAP at the indicated time points. (B) Memory differentiation phenotypes of RhPAP-specific T cells responding to peptide pools in each RM were determined on the basis of CD28 and CCR7 expression, delineating central memory (T_CM, CD28⁺, CCR7⁺), transitional effector-memory (T_TrEM, CD28⁺, CCR7⁻), and effector-memory (T_EM, CD28⁻, CCR7⁻). (C) CD8⁺ T cell responses and MHC restriction to individual 15–amino acid oligomer peptides from the RhPAP mix were determined as in Fig. 3. All peptide-specific responses were MHC-Ia restricted (shown in red color), i.e., they were blocked by pan–MHC-I antibody but not by VL9 peptide or anti–MHC-II antibodies. Results for each RM in (A) and (B) and overall analysis of 15–amino acid oligomer peptide responses in (C) are listed in data file S3.