Fig. 1. Female mice display higher susceptibility to anxiety than male mice after acute exposure to forced swimming stress.

(A) Experimental timeline for measuring anxiety-like behaviors following 5-min treatment of forced swimming. (B) Locomotion traces for representative control and forced swimming-exposed female and male mice in EPM. Light gray marks closed arms. (C and D) Quantification of frequency to open arms (C) and percentage time spent in the open arms (D) of EPM in female and male mice (*P < 0.05 and **P < 0.01, unpaired t test). (E) Locomotion traces for representative control and forced swimming-exposed female and male mice in OFT. Light gray square marks the designated center zone. (F and G) Quantification of frequency to center zone (F) and percentage time spent in the center zone (G) of OFT in female and male mice (**P < 0.01, unpaired t test).