Table 1.
FITC | PE | PerCP Cy5.5 | PE-Cy7 | APC | APC-H7 | |
---|---|---|---|---|---|---|
Tube 1 (unstained) | Blank | Blank | Blank | Blank | Blank | CD 45 (3 µL) |
Tube 2 (B-Tube) | CD 20 (10 µL) | CD 10 (10 µL) | CD 38 (10 µL) | CD 19 (3 µL) | CD 34 (3 µL) | CD 45 (3 µL) |
Tube 3 (T-Tube) | CD 8 (10 µL) | CD 5 (10 µL) | CD 3 (3 µL) | CD 4 (3 µL) | CD 7 (3 µL) | CD 45 (3 µL) |
Tube 4 (M-Tube) | CD 64 (10 µL) | CD 33 (10 µL) | HLA-DR (10 µL) | CD 13 (3 µL) | CD 117 (3 µL) | CD 45 (3 µL) |
Tube 5 (Cytoplasmic Tube) | cMPO (10 µL) (added later) | CD 79a (10 µL) (added later) | CD 3 (10 µL) (added later) | Blank | CD 34 (3 µL) | CD 45 (3 µL) |