Figure 1.

Identification of immunogenic HLA‐B*18:01 predicted peptides. PBMCs from HLA‐B*18:01⁺ individuals (n = 5) were stimulated with a pool of peptides (2 μm per peptide) for 10+ days to generate pooled CD8⁺ T cell lines. CD8⁺ T cells were stimulated with the peptide pool (2 μm) and each peptide individually (10 μm), along with negative (no peptide) and positive (×500) controls, and responses were assessed using an ICS assay. (a) Representative FACS plots from donor SG115. (b) A bar graph outlining the average proportion of IFN‐γ⁺ of CD8⁺ T cells, minus the no peptide control, in five HLA‐B*18:01⁺ donors, with each coloured dot representing a donor's response (red, SG10; green, SG26; yellow, SG112; blue, SG115; purple, SG131).