FIG. 5.
Downregulation of MR in J774E cells is associated with reduced susceptibility to influenza virus infection. (A) J774E cells were cultured for 2 h in the presence (◊) or absence (⧫) of 25 mM d-mannose, then washed free of the sugar and assayed for 125I-mBSA binding. Scatchard plots of the data (inset) yielded Kd values of 10 and 11.1 nM for cells cultured in the presence or absence of mannose, respectively. (B) J774E cell monolayers in chamber slides were incubated for 2 h in the presence (□) or absence (■) of 25 mM d-mannose and then infected for 1 h with increasing doses of BJx109. The presence or absence of mannose was maintained, as appropriate, throughout all stages of the infection assay including virus adsorption, washing, and subsequent culture steps. The results given are from one of two similar experiments.