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The YY1 binding site is at the 5′ end of the E1 promoter. (A) Fragments used to dissect the E1 promoter for the YY1 binding site(s) are shown. The nucleotide coordinates of E1p and E1a are provided in the legend to Fig. 1. E1c extends from nt 528 to 585; E1d extends from nt 586 to 670. (B) EMSAs were conducted using radiolabeled E1p and unlabeled E1p subfragments as competitors. The C1 complex contains CDP, most of the binding activity of which is located in E1a (1).
