Fig. 4.

XAV939 (degradation of β-catenin) attenuated the effect of atorvastatin on cultured microglia. A The protein levels of β-catenin were detected by the capillary western blot test, n = 3/group. B Representative photographs of double immunofluorescence staining of CD16 (red) and Iba1 (green). Scale bar = 50 μm, n = 6/group. C Representative photographs of double immunofluorescence staining of CD206 (red) and Iba1 (green). Scale bar = 50 μm, n = 6/group. D, F The mRNA levels of Inos, Tnfα, Il6, Arg1, Tgfb, and Mrc1 were detected by RT-qPCR, n = 3/group. E, G The cell culture supernatant protein levels of iNOS, TNFα, ARG1, and TGFβ were detected by ELISA, n = 3/group. Values represent the mean ± SEM. ^*p < 0.05, ^**p < 0.01 vs. the respective control