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. 2023 Nov 24;61(6):3559–3577. doi: 10.1007/s12035-023-03777-y

Table 1.

Oligonucleotide primers and PCR conditions used for real-time quantitative PCR

Gene Forward primer Reverse primer Product (bp) Annealing (°C)
Rat
Il6 ATTCTGTCTCGAGCCCACCA CTGAAGGGCAGATGGAGTTGA 51 60
Inos AGATCCCGAAACGCTACACTT TGCGGCTGGACTTCTCACTC 174 60
Tnfα GCATGATCCGAGATGTGGAACTGG CGCCACGAGCAGGAATGAGAAG 113 60
Arg1 ACATCAACACTCCGCTGACAACC GCCGATGTACACGATGTCCTTGG 152 60
Mrc1 GAGGACTGCGTGGTGATGAA CATGCCGTTTCCAGCCTTTC 154 60
Tgfb CTGCTGACCCCCACTGATAC AGCCCTGTATTCCGTCTCCT 94 60
β-actin ACCCGCCACCAGTTCGC CACGATGGAGGGGAAGACG 123 60

IL6 interleukin 6, Inos inducible nitric oxide synthase, Tnfα tumor necrosis factor α, Arg1 arginase 1, Mrc1 mannose receptor C-type 1, Tgfb transforming growth factor β