Table 1.
Gene | Forward primer | Reverse primer | Product (bp) | Annealing (°C) |
---|---|---|---|---|
Rat | ||||
Il6 | ATTCTGTCTCGAGCCCACCA | CTGAAGGGCAGATGGAGTTGA | 51 | 60 |
Inos | AGATCCCGAAACGCTACACTT | TGCGGCTGGACTTCTCACTC | 174 | 60 |
Tnfα | GCATGATCCGAGATGTGGAACTGG | CGCCACGAGCAGGAATGAGAAG | 113 | 60 |
Arg1 | ACATCAACACTCCGCTGACAACC | GCCGATGTACACGATGTCCTTGG | 152 | 60 |
Mrc1 | GAGGACTGCGTGGTGATGAA | CATGCCGTTTCCAGCCTTTC | 154 | 60 |
Tgfb | CTGCTGACCCCCACTGATAC | AGCCCTGTATTCCGTCTCCT | 94 | 60 |
β-actin | ACCCGCCACCAGTTCGC | CACGATGGAGGGGAAGACG | 123 | 60 |
IL6 interleukin 6, Inos inducible nitric oxide synthase, Tnfα tumor necrosis factor α, Arg1 arginase 1, Mrc1 mannose receptor C-type 1, Tgfb transforming growth factor β