Fig. 3.

Neurodegenerative state in Epm2b − / − mice and the therapeutic efficacy of fingolimod and dimethyl fumarate treatments on the hindlimb clasping test. A Representative pictures of the three main phenotypes observed (absent, mild, and moderate). B Relative frequency distribution of the hindlimb clasping score representing the severity of neurodegenerative signs (see Material and Methods section). Frequency histograms show frequency distribution among 4 scores: absent, mild, moderate, and severe. Statistical differences between groups were analyzed by Pearson’s chi-square test or by Fisher’s exact test when sample sizes were zero. Statistical significance was defined as **P < 0.01 and.^####P < 0.0001 (WT-water n, 10; Epm2b-/- water n, 10; Epm2b-/- fingolimod n, 16; Epm2b-/- dimethyl fumarate n, 14) (see Supplementary Table S1)