Table 2.
IHC expression among the groups.
| Antibody | Experimental | Comparative | A.B p-value | ||||
|---|---|---|---|---|---|---|---|
| Min. H score | Max. H score | Mean ± SD (A) | Min. H score | Max. H score | Mean ± SD (B) | ||
| ATF-6 | 31.46 | 150.25 | 80.07 ± 27.84 | 33.77 | 125.88 | 77.46 ± 38.89 | 0.881 |
| PERK | 54.47 | 117.41 | 92.16 ± 18.27 | 61.49 | 141.90 | 91.00 ± 9.81 | 0.935 |
| XBP1 | 34.32 | 86.13 | 63.09 ± 14.91 | 30.02 | 105.58 | 65.41 ± 31.13 | 0.840 |
| NFκB | 57.54 | 145.87 | 126.33 ± 29.96 | 167.66 | 176.15 | 163.71 ± 12.94 | 0.029* |
| GADD153 | 76.99 | 199.47 | 106.48 ± 35.31 | 60.91 | 155.44 | 104.30 ± 47.82 | 0.921 |
| MLKL | 135.58 | 173.54 | 156.71 ± 12.82 | 128.37 | 192.058 | 158.32 ± 26.09 | 0.862 |
| LC3 | 95.18 | 166.66 | 119.44 ± 23.71 | 84.48 | 162.32 | 124.90 ± 33.48 | 0.722 |
| E-cadherin | 29.94 | 125.18 | 78.12 ± 30.52 | 33.91 | 125.72 | 71.61 ± 38.79 | 0.734 |
| TGF-β | 4.95 | 90.12 | 44.23 ± 26.86 | 13.50 | 54.12 | 32.47 ± 17.98 | 0.429 |
| αSMA | 1.44 | 127.81 | 47.32 ± 41.45 | 13.80 | 111.51 | 64.00 ± 49.49 | 0.510 |
| HIF | 0.37 | 48.60 | 15.69 ± 20.49 | 7.53 | 19.50 | 13.52 ± 8.47 | 0.892 |
| IL-8 | 1.73 | 25.23 | 8.03 ± 9.75 | 16.56 | 98.77 | 57.67 ± 58.13 | 0.083 |
H scores were calculated with Qupath® (Northern Ireland Molecular Pathology Laboratory, Centre for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast, United Kingdom). H score interpretation: < 10 (negative), 10–100 (low), 101–200 (moderate), > 200 (intense). Student-t test was performed for average score of H score with *p value < 0.05 was considered statistically significant.
All antibodies were obtained from Santa Cruz Biotechnology, USA. *αSMA, α-smooth muscle actin; ATF6, activating transcription factor 6; CHOP/GADD153, CCAAT-enhancer-binding protein homologous protein/growth arrest-and DNA damage-inducible gene 153; E-cadherin, epithelial cadherin; HIF-1, hypoxia-inducible factor 1; IL-8, interleukin 8; LC3, microtubule-associated protein 1 light chain 3; MLKL. Mixed lineage kinase domain-like protein; NFκB, nuclear factor kappa-light-chain-enhance of activated B cells; PERK, protein kinase RNA activated (PKR-like ER kinase); TGFβ, transforming growth factor beta; XBP1, xbox-binding protein 1.