Fig. 4. TFPI interacts with Thbd in F4/80⁺CD169⁺Vcam-1⁺ macrophages.

A mRNA expression of TF in F4/80⁺CD169⁺Vcam-1⁺ macrophages of Jak2^V617F-mutated or hypoxia-exposed mice (n = 5). B TF procoagulant activity and plasma TAT levels in mice after TF mAb treatment (n = 5). C Heme content in F4/80⁺CD169⁺Vcam-1⁺ macrophages of TFPI^f/f;EpoR mice after TF mAb treatment (n = 5). D Fech mRNA expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages of TFPI^f/f;EpoR mice after TF mAb treatment (n = 5). E PB Hb in TFPI^f/f;EpoR mice after TF mAb treatment (n = 5). F Co-IP analysis of the interaction between TFPI and Thbd in HEK293T cells (n = 2). G Schematic illustration of Thbd and TFPI constructs. H Co-IP analysis of the interaction of TFPI with the different domains of Thbd in HEK293T cells (n = 2). I Co-IP analysis of the interaction between TFPI and Thbd in F4/80⁺CD169⁺Vcam-1⁺ macrophages (n = 2). J Pull down analysis of the interaction between the TFPI and Thbd (n = 2). K Thbd protein expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages after Thbd shRNA treatment (n = 3). L Fech mRNA expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages after Thbd shRNA and rTFPI treatment (n = 5). M Fech mRNA expression in HEK293T cells after Thbd transfection and rTFPI treatment (n = 5). Statistical analysis was performed using one-way ANOVA (B–E and K–M). Data are shown as mean ± SEM and are representative of two (B–J) or three (A and K–M) independent experiments. Source data are provided as a Source Data file.