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. 2024 May 10;15:3976. doi: 10.1038/s41467-024-48328-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A mRNA expression of aPC in F4/80⁺CD169⁺Vcam-1⁺ macrophages of Jak2^V617F-mutated and hypoxia-exposed mice (n = 5). B aPC protein expression of F4/80⁺CD169⁺Vcam-1⁺ macrophages after intraosseous infusion of lentivirus-expressing aPC shRNA (n = 3). C Frequency of RIII, RIV, and RV erythroblast populations in Thbd^f/f;LysM mice after intraosseous infusion of lentivirus-expressing aPC shRNA (n = 5). D KEGG analysis of downregulated genes in F4/80⁺CD169⁺Vcam-1⁺ macrophages in TFPI^f/f;EpoR mice. E Heme content in F4/80⁺CD169⁺Vcam-1⁺ macrophages treated with rTFPI combined with JNK, FOXO, and ERK1/2 inhibitors (n = 5). F Heme content in F4/80⁺CD169⁺Vcam-1⁺ macrophages after treated with rTFPI combined with ERK1/2 inhibitor and GATA1 shRNA (n = 10). G Phosphorylation level of ERK1/2 protein in F4/80⁺CD169⁺Vcam-1⁺ macrophages after rTFPI treatment (n = 3). H Phosphorylation level of GATA1 protein in F4/80⁺CD169⁺Vcam-1⁺ macrophages of Thbd^f/f;LysM mice after rTFPI treatment (n = 3). I Phosphorylation level of GATA1 protein in F4/80⁺CD169⁺Vcam-1⁺ macrophages treated with rTFPI and ERK1/2 inhibitor (n = 3). J Phosphorylation level of GATA1 protein in F4/80⁺CD169⁺Vcam-1⁺ macrophages of Thbd^f/f;LysM mice at different time points after rTFPI treatment (n = 3). K ALAS2 and Fech mRNA expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages of Thbd^f/f;LysM mice after rTFPI treatment (n = 5). L ALAS2 and Fech mRNA expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages after treated with rTFPI and ERK1/2 inhibitor (n = 5). M ALAS2 and Fech mRNA expression in F4/80⁺CD169⁺Vcam-1⁺ macrophages after treated with rTFPI and GATA1 shRNA (n = 5). Statistical analysis was performed using one-way ANOVA (B, C, E, F, and K–M). Data are shown as mean ± SEM and are representative of two (C, E, and F) or three (A, B, and G–M) independent experiments. N The signaling pathway of TFPI/Thbd mediated heme synthesis in F4/80⁺CD169⁺Vcam-1⁺ macrophages. Source data are provided as a Source Data file.