Fig. 4. MAGMA-seq infers biophysical properties in mixed antibody, mixed antigen sorts.
a Antigen-specific antibody sub-libraries and antigens used in the sorting experiments. Non-binders 1G01 and 1G04 were also included. b Probability of an antibody variant sorted into an antigen-specific bin when only 2000 nM of S1 (Y-axis) or H1 HA (X-axis) was incubated with yeast. For HA, only the top sorting bin was included in the analysis. c R2 of MLE KD estimates from sort replicates for HA targeting antibodies 222-1C06, 319–345 (anchor epitope), and CR6261 (stem epitope). d Structure (PDB ID: 3GBN) of CR6261 bound to H1 HA. Purple sticks are HA-contacting positions that are encoded from the inferred UCA sequence. The side chains of residues mutated in the mature antibody relative to the UCA are shown as gold sticks and lines. e, f LASSO regression of e logKD ratios and f Fmax percentage differences one body and two body weights for CR6261. Weights are shown relative to the mature antibody. Source data are provided as a Source Data file.