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. 2024 May 10;15:3969. doi: 10.1038/s41467-024-48287-0

Fig. 4. WWP2-dependent IFN responses in iPSCs-derived neural stem cells (NSCs).

Fig. 4

Induction of IFNβ, ISG56 and Mx1 mRNA after different time of Poly(I:C) stimulation in neural precursor cells (A), neurons (B) and astrocytes (C) from WWP2 p.R841 individuals [black, N = 5 (A), N = 4 (B), N = 5 (C)] and from WWP2 p.R841H individuals [red, N = 8 (A, B), N = 4 (C)]. As a control, the expression of IFNβ was measured in neural precursor cells (D), neurons (E) and astrocytes (F) from WWP2 p.R841 individuals (black) and from WWP2 p.R841H individuals (red), stimulated with LPS [WWP2 p.R841 individuals N = 5 (D, E), N = 4 (F); WWP2 p.R841H individuals N = 8 (D, E), N = 2 (F)], CPG [WWP2 p.R841 individuals N = 5 (D, E), N = 4 (F); WWP2 p.R841H individuals N = 8 (D, E), N = 2 (F)] and R848 [WWP2 p.R841 individuals N = 5 (D, E), N = 4 (F); WWP2 p.R841H individuals N = 8 (D, E), N = 2 (F)] for 2, 3 and 4 h. A.U. stands for arbitrary unit. Results represent the mean ± standard error of 1 representative experiment among 2 (A, B), 3 (C), and 1 (D–F). Statistical analyses were performed using an unpaired two-tailed Student t-test. A Left *, **, *** mean for P = 0.0129, P = 0.0011 and P = 0.0001, (A) middle *, ** mean for P = 0.0501 and P = 0.0413, (A) right *, **, *** mean for P = 0.0010, P = 0.0025, P = 0.0021. B Left *, **, *** mean for P = 0.0026, P = 0.0006 and P = 0.0187, (B) middle *, ** mean for P = 0.0299 and P = 0.0022. D * means for P = 0.0475. E *, ** mean for P = 0.0121 and P = 0.0005.