Fig. 3. Post-translational modifications of FgTad3.

a Western blots of total proteins isolated from 24 h hyphae (Hyp) and 7-dpf perithecia (Sex) of the transformant ectopically expressing FgTad3-GFP, probed with anti-GFP antibodies. b MS/MS spectrum of acetylated and phosphorylated peptides. acK¹⁹⁸ and pE²⁴¹ indicate the location of acetylation and phosphorylation, respectively. c Morphology of colonies, perithecia, and asci/ascospores of the modification-mimetic and modification-deficient mutants for the acK¹⁹⁸ and pE²⁴¹ of FgTAD3. White bar = 0.2 mm; black bar = 20 μm. d Cartoon model depicting the location of K¹⁹⁸ and E²⁴¹ sites in FgTad3.