Table 2.

Site-directed mutagenesis specifications

Variant name	Sequence changea	Fwd. primer	Rev. primer	Annealing temperature (°C)
Kozak_hPMS2	N/A	Univ_F	WT_R	59
Kozak_Var01	c.1A>C	Kozak_Var01_F	Kozak_Var01_R	60
Kozak_Var02	c.−1C>G	Kozak_Var02_F	Kozak_Univ01_R	63
Kozak_Var03	c.−1C>T	Kozak_Var03_F	Kozak_Univ01_R	63
Kozak_Var04	c.−1C>A	Kozak_Var04_F	Kozak_Univ01_R	63
Kozak_Var05	c.−2C>G	Kozak_Var05_F	Kozak_Var05_R	65
Kozak_Var06	c.−2C>A	Kozak_Var06_F	Kozak_Univ02_R	63
Kozak_Var07	c.−2C>T	Kozak_Var07_F	Kozak_Univ02_R	63
Kozak_Var08	c.−3T>G	Kozak_Var08_F	Kozak_Var08_R	65
Kozak_Var09	c.−4A>G	Kozak_Var09_F	Kozak_Var09_R	63
Kozak_Var10	c.−5C>G	Kozak_Var10_F	Kozak_Univ03_R	61
Kozak_Var11	c.−5C>A	Kozak_Var11_F	Kozak_Univ03_R	61
Kozak_Var12	c.−6G>A	Kozak_Var12_F	Kozak_Univ04_R	57
Kozak_Var13	c.−9G>C	Kozak_Var13_F	Kozak_Var13_R	57
Kozak_Var14	c.−7_−5del	Kozak_Var14_F	Kozak_Var14_R	63
Kozak_Var15	c.−8_−6del	Kozak_Var15_F	Kozak_Var15_R	61
Kozak_Var16	c.−6G>C	Kozak_Var16_F	Kozak_Univ04_R	57
Kozak_Var17	c.−7T>C	Kozak_Var17_F	Kozak_Var17_R	58

Fwd., forward; Rev., reverse.

^aAnnotations on NM_000535.7(PMS2).