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. 2024 Apr 30;121(19):e2317703121. doi: 10.1073/pnas.2317703121

Fig. 1.

Fig. 1.

Fixation-driven chemical cross-linking strategy enables PKMO FX to achieve efficient fluorescence signal retention. (A) Chemical structure of Cy3.5-NH2 (PKMO FX). (B) Comparison of different fixable mitochondrial dyes after 2% GA fixation. Cy3.5-NH2 shows the highest Signal-to-background ratio (SBR) and mitochondrial fluorescence retention (MFR) after fixation. It has the best STED compatibility among commercial and designed mitochondrial dyes in this study. Abbreviations: MTR (MitoTracker Red CMXRos); MTDR (MitoTracker Deep Red FM). (C) Normalized absorption and fluorescence spectra of PKMO FX. Ex = 584 nm, Em = 604 nm.