Fig. 5.

Analysis of exosomes released from neuron-free muscle and innervated muscle. (A) Number of exosomes in the conditioned media normalized to intracellular proteins after 24-h incubation (n = 3, *P < 0.05, **P < 0.01). (B) Normalized read counts of the top 95% abundant exosomal miRNAs. (C) miRNA-target gene network of the top 95% miRNAs of (C, I) Neuron-free Muscle and (C, II) Innervated Muscle(G). Genes targeted by at least two miRNAs were selected for the network analysis. Genes related to nervous system development were marked with a yellow circle, and muscle development-related genes were marked with red circles. Genes regulating both nerve and muscle development were marked with green circles. (D) Enriched nervous system and muscle development-related biological processes and (E) Enriched KEGG pathways regulated by the top 95% miRNA in the exosomes secreted by Neuron-free Muscle or Innervated Muscle(G). (F) Heat map showing the fold change of top 95% exosomal miRNA expression between the Neuron-free Muscle and Innervated Muscle(G) (P-value < 0.05, n = 3). The color key indicates the fold change of miRNA expression; red indicates upregulation, and blue indicates downregulation.