Fig. 3.

Characteristics of the “unbiased” NC clusters c1-c5-c14. (A) Expression of pluripotency (cmyc, sox2, pou5f3.1) and early unbiased NC (tfap2b, c3, c9, sox9, pax3, zic1, tfacp2c) genes in clusters c1, c5, c14, c11, c15. Each bar is a bin of 40 cells. The “stage” line indicates cells’ developmental stage, the “dpt-pseudotime” cell advancement along their transcriptional path. (B) Highly significant differential expression of Pou5f1-1 and sox2 in clusters c1-c5 (early stage, high expression), c14 (mid-neurula stage, moderate expression) and c11-c15 (late stage, low expression). (C) Sustained expression of unbiased NC markers tfap2b and cmyc and pluripotency marker pou5f3.1 in microdissected NB (stage 14), NC (stages 16 to 18) and whole embryos (stage 18). AS—antisense probe, S—sense probe control.