Fig. 3.

UNC-68/RYR and ITR-1/IP3R are both required for VICR-mediated SLO-2 activation. Ik_hiCl currents were recorded from adult body wall muscles at holding potentials of −60 to +60 mV in the presence and absence of external calcium. Representative Ik_hiCl traces are shown (A). The ratio of Ik_hiCl current density at +30 mV recorded in the absence of external calcium to the mean current density recorded in 5 mM Ca²⁺ [Ik_hiCl (0/5Ca²⁺)] is plotted for each genotype (B). The Ik_hiCl (0/5Ca²⁺) ratio was significantly decreased in unc-68 and itr-1(mKO) single mutants and was not further reduced in the corresponding double mutant. These results suggest that UNC-68 and ITR-1 function together to promote SLO-2 activation. Values that differ significantly are indicated (ns, not significant; **P < 0.01; ****P < 0.0001). Error bars indicate SEM.