Fig. 6.

ImmTAB-inhA mediates antigen-dependent T cell killing of target cells. (A) Apoptosis of Ag+ HEK293T cells cocultured with PBMC and a titration of ImmTAB-inhA measured by IncuCyte. Controls include Ag+ HEK 293T in the presence of ImmTAB-inhA with or without cognate peptide and Ag- HEK 293T in the presence of a CD3 nonbinding version of ImmTAB-inhA (anti-CD3^mut ImmTAB-inhA). Data are representative from one of three donors tested in triplicate. (B) Confocal imaging of PBMC (blue) cocultured with a mixture of Ag+ (red; transduced with full-length inhA) and Ag− (yellow; nontransduced) A549 cells in the presence or absence of ImmTAB-inhA corresponding to Movies S1 and S2, respectively. Arrows indicate apoptosis of Ag+ target cells.