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The effectiveness of Zeocin selection on helper virus gene expression in AMIZ cells over time. (A) Unspliced MoMLV transcript (gag-pol-env-IRES-Zeo) and spliced RNA (env-IRES-Zeo) were detected by an env probe in cellular RNA extracted from AMIZ cells with and without continuous Zeocin selection on days 0, 15, 54, and 78. (B) Hybridization with the GAPDH cDNA probe demonstrates the relative RNA loading.
