Fig. 14.

T-help and T-Cyto T cell infiltration. (A) Representative IHC images for CD4 (T-help T cell marker) and CD8 (T-Cyto T cell marker) showing T cell infiltration and the phenotype of the infiltrated T cells in the different hydrogels at days 3, 7, and 14 postsubcutaneous injections. DAPI was used to label the cellular nuclei (blue). (B) Ratio calculations of T-help/T-Cyto using the percentages of CD4+ (T-help) and CD8+ (T-Cyto) T cells as measured by flow cytometry. (C) Ratio calculations of Th2/Th1 using the percentages of IL-4Rα+ (Th2) and IFN-γ+ (Th1) T cells as measured by flow cytometry. (Scale bar, 50 µm.)