The protein expression of NLRP3, Caspase-1, and P2X7 in rat gastric smooth muscle cells, and ATP content in the cell culture supernatants. (a) Western blot analysis of NLRP3, Caspase-1, and P2X7 expression; (b) ELISA detection of ATP content. Data are expressed as mean ± standard error of the mean, t-test and one-way ANOVA were used for comparisons between groups, n = 3 per group. *P < 0.05, **P < 0.01. Abbreviations used: ATP, adenosine triphosphate; NLRP3, nucleotide-binding oligomerization domain-like receptor 3.